Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
Blog Article
Yili Qu1,3, Ping Wang1,3, Yi Man1, Yubao Li2, Yi Zuo2, Jidong Li21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China; 2Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China; 3These authors contributed equally to this workAbstract: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) composite with good bioactivity and osteoconductivity was employed to develop a novel porous membrane with asymmetric structure for guided bone regeneration (GBR).In order to test material cytotoxicity and to investigate surface-dependent responses of bone-forming cells, the morphology, proliferation, and cell cycle of bone marrow stromal cells (BMSCs) of ghost_product rats cultured on the prepared membrane were determined.The polygonal and fusiform shape of BMSCs was observed by scanning electronic microscopy (SEM).The proliferation of BMSCs cultured on nHA/PA66 membrane tested by the MTT method (MTT: [3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazoliumbromide]) was higher than that of negative control groups for 1 and 4 days’ incubation and had no significant difference for 7 and 11 days’ culture.
The results of cell cycle also suggested that the membrane has no negative influence on cell division.The nHA/PA66 membranes were then implanted into subcutaneous sites of nine Sprague Dawley rats.The wounds and implant sites were free from suppuration and necrosis in all periods.All nHA/PA66 membranes were surrounded by a fibrous capsule with Playing Cards decreasing thickness 1 to 8 weeks postoperatively.
In conclusion, the results of the in vitro and in vivo studies reveal that nHA/PA66 membrane has excellent biocompatibility and indicate its use in guided tissue regeneration (GTR) or GBR.Keywords: hydroxyapatite/polyamide, barrier membrane, biocompatibility, guided bone regeneration.